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  • P-ISSN2765-2203
  • E-ISSN2765-2211

Dietary Risk Assessment of Snf7 dsRNA for Coccinella septempunctata

Proceedings of the National Institute of Ecology of the Republic of Korea, (P)2765-2203; (E)2765-2211
2021, v.2 no.3, pp.210-218
https://doi.org/10.22920/PNIE.2021.2.3.210
Young Jun Jung (Division of Ecological Safety, National Institute of Ecology)
Min-A Seol (Division of Ecological Safety, National Institute of Ecology)
Wonkyun Choi (Division of Ecological Safety, National Institute of Ecology)
Jung Ro Lee (Division of Ecological Safety, National Institute of Ecology)

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Abstract

Recently, pest-resistant living modified (LM) crops developed using RNA interference (RNAi) technology have been imported into South Korea. However, the potential adverse effects of unintentionally released RNAi-based LM crops on non-target species have not yet been reported. Coccinella septempunctata, which feeds on aphids, is an important natural enemy insect which can be exposed to the double-stranded RNA (dsRNA) produced by RNAi-based LM plants. To assess the risk of ingestion of Snf7 dsRNA by C. septempunctata, we first identified the species through morphological analysis of collected insects. A method for species identification at the gene level was developed using a specific C. septempunctata 12S rRNA. Furthermore, an experimental model was devised to assess the risk of Snf7 dsRNA ingestion in C. septempunctata. Snf7 dsRNA was mass-purified using an effective dsRNA synthesis method and its presence in C. septempunctata was confirmed after treatment with purified Snf7 dsRNA. Finally, the survival rate, development time, and dry weight of Snf7 dsRNA-treated C. septempunctata were compared with those of GFP and vATPase A dsRNA control treatments, and no risk was found. This study illustrates an effective Snf7 dsRNA synthesis method, as well as a high-concentration domestic insect risk assessment method which uses dsRNA to assess the risk of unintentional released of LM organisms against non-target species.

Submission Date
2021-06-04
Revised Date
2021-06-29
Accepted Date
2021-06-30

Proceedings of the National Institute of Ecology of the Republic of Korea