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  • P-ISSN2733-7243
  • E-ISSN2734-1372

Identification of Molecular Markers for Population Diagnosis of Korean Fir (Abies koreana) Vulnerable to Climate Change

Proceedings of the National Institute of Ecology of the Republic of Korea, (P)2733-7243; (E)2734-1372
2020, v.1 no.1, pp.68-73
https://doi.org/10.22920/PNIE.2020.1.1.68
Dong Wook Kim (National Institute of Ecology)
Da Young Park (National Institute of Ecology)
Dae Young Jeong (National Institute of Ecology)
Hyeong Cheol Park (National Institute of Ecology)

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Abstract

Korean fir (Abies koreana) is an evergreen coniferous tree species that is unique to South Korea. A. koreana is found in a limited sub-alpine habitat and is considered particularly vulnerable to climate change. Identification of populations vulnerable to climate change is an important component of conservation programs. In this study, a heat stress-induced transcriptome RNA-seq dataset was used to identify a subset of six genes for assessment as candidate marker genes for ecologically vulnerable populations. Samples of A. koreana were isolated from ecologically stable and vulnerable regions of the Halla and Jiri mountains, and the expression levels of the six candidate markers were assessed using quantitative real-time polymerase chain reaction. All six of the candidate genes exhibited higher expression levels in samples from vulnerable regions compared with stable regions. These results confirm that the six high temperature-induced genes can be used as diagnostic markers for the identification of populations of A. koreana that are experiencing stress due to the effects of climate change.

Submission Date
2020-09-18
Revised Date
2020-10-06
Accepted Date
2020-10-07

Proceedings of the National Institute of Ecology of the Republic of Korea